Frosina beat Roma in the Serie A derby after a second-half brace from Alessandro Del Piero and a penalty shootout victory from Giuseppe Meazza.
Mozambique’s Guinean striker David de Laet scored a brace to earn the Giallorossi a comfortable 2-0 win over Roma in their opening fixture.
Roma won for the first time in 13 league matches, but Del Pieroe added an early penalty in stoppage time.